Modified colorimetric ultramicro method for estimating Nefa in serum

M. C. Elphick

Institute of Child Health, University of London

A method is described for the estimation of serum free fatty acids based on the ultramicro method of Novák. The difficulty of obtaining an accurate standard graph has been overcome and the interfering effects of phospholipids have been reduced. The method is simple to perform and gives accurate, precise results in 50 µl samples; it is thus suitable for work on newborn babies and small laboratory animals.

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