Proliferative activity in endometrial stromal granulocytes throughout menstrual cycle and early pregnancy.

D Pace, L Morrison, J N Bulmer

Department of Pathology, University of Leeds.

The proliferative activity in endometrial stromal granulocytes was studied using two approaches. Firstly, mitotic activity was studied in paraffin wax embedded sections of normal non-pregnant endometrium and early pregnancy decidua stained with phloxine-tartrazine. Secondly, the monoclonal antibody Ki67 was applied to cryostat sections of similar tissues. Endometrial stromal granulocytes were identified by their labelling with NKH1, Dako T11, UCHL1 or Dako-LC. The percentage of cases in which endometrial stromal granulocytes showed mitosis was 25%, 75%, 86%, and 93%, respectively in proliferative, early secretory, mid secretory, and late secretory phases, and 14% in early decidua. There were at most one or two endometrial stromal granulocytes in mitosis per 10 high power fields. Double labelled cells were present in small numbers in proliferative endometrium and in moderate numbers in secretory endometrium. Only a few cells in early decidua double labelled with Ki67/T11; moderate numbers of cells double labelled with Ki67/Dako-LC. It is concluded that proliferative activity does occur in endometrial stromal granulocytes and is particularly prominent in the late secretory phase.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Manaster, I., Mizrahi, S., Goldman-Wohl, D., Sela, H. Y., Stern-Ginossar, N., Lankry, D., Gruda, R., Hurwitz, A., Bdolah, Y., Haimov-Kochman, R., Yagel, S., Mandelboim, O. (2008). Endometrial NK Cells Are Special Immature Cells That Await Pregnancy. J. Immunol. 181: 1869-1876 [Abstract] [Full Text]  
• Tuckerman, E., Laird, S.M., Prakash, A., Li, T.C. (2007). Prognostic value of the measurement of uterine natural killer cells in the endometrium of women with recurrent miscarriage. Hum Reprod 22: 2208-2213 [Abstract] [Full Text]  
• Lynch, L., Golden-Mason, L., Eogan, M., O'Herlihy, C., O'Farrelly, C. (2007). Cells with haematopoietic stem cell phenotype in adult human endometrium: relevance to infertility?. Hum Reprod 22: 919-926 [Abstract] [Full Text]  
• Ponnampalam, A. P., Weston, G. C., Trajstman, A. C., Susil, B., Rogers, P. A.W. (2004). Molecular classification of human endometrial cycle stages by transcriptional profiling. Mol Hum Reprod 10: 879-893 [Abstract] [Full Text]  
• Chao, K.-H., Wu, M.-Y., Yang, J.-H., Chen, S.-U., Yang, Y.-S., Ho, H.-N. (2002). Expression of the interleukin-2 receptor {alpha} (CD25) is selectively decreased on decidual CD4+ and CD8+ T lymphocytes in normal pregnancies. Mol Hum Reprod 8: 667-673 [Abstract] [Full Text]  
• Okada, H., Nakajima, T., Sanezumi, M., Ikuta, A., Yasuda, K., Kanzaki, H. (2000). Progesterone Enhances Interleukin-15 Production in Human Endometrial Stromal Cells in Vitro. J. Clin. Endocrinol. Metab. 85: 4765-4770 [Abstract] [Full Text]  
• Verma, S., Hiby, S. E., Loke, Y. W., King, A. (2000). Human Decidual Natural Killer Cells Express the Receptor for and Respond to the Cytokine Interleukin 15. Biol. Reprod. 62: 959-968 [Abstract] [Full Text]  
• Croy, B. A., Ashkar, A. A., Minhas, K., Greenwood, J. D. (2000). Can Murine Uterine Natural Killer Cells Give Insights Into the Pathogenesis of Preeclampsia?. Reproductive Sciences 7: 12-20 [Abstract]  
• Okada, S., Okada, H., Sanezumi, M., Nakajima, T., Yasuda, K., Kanzaki, H. (2000). Expression of interleukin-15 in human endometrium and decidua. Mol Hum Reprod 6: 75-80 [Abstract] [Full Text]  
• Habiba, M.A., Bell, S.C., Al-Azzawi, F. (1999). The effect of hormone replacement therapy on the number and the proliferation index of endometrial leukocytes. Hum Reprod 14: 3088-3094 [Abstract] [Full Text]  
• Wahab, M., Thompson, J., Al-Azzawi, F. (1999). The distribution of endometrial leukocytes and their proliferation markers in trimegestone-treated postmenopausal women compared to the endometrium of the natural cycle: a dose-ranging study. Hum Reprod 14: 1201-1206 [Abstract] [Full Text]  
• King, A., Gardner, L., Loke, Y.W. (1999). Co-stimulation of human decidual natural killer cells by interleukin-2 and stromal cells. Hum Reprod 14: 656-663 [Abstract] [Full Text]