FOXP3 immunohistochemistry on formalin-fixed paraffin embedded tissue- poor correlation between different antibodies
Yin Ling Woo 1*, Jane C Sterling 1, Robin AF Crawford 2, Sjoerd H van der Burg 3, Nicholas Coleman 1 and Margaret A Stanley 1
1 University of Cambridge, United Kingdom
2 Addenbrooke's Hospital, United Kingdom
3 Leiden University Medical Centre, Netherlands
* To whom correspondence should be addressed. E-mail: ylw22{at}cam.ac.uk.
Accepted 13 March 2008
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Abstract |
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Since its original description, there has been a substantial output of publications related to the FOXP3 gene. FOXP3 protein, a member of the forkhead/winged-helix family of transcriptional regulators is a nuclear product and is not expressed in the cell cytoplasm or surface. Expression of this single transcription factor causes a developmental switch in naïve T-cells to a suppressor cell phenotype, more commonly referred to as regulatory T-cells (Tregs). Tregs are now intensively studied in various autoimmune diseases, infections and different cancers. An increasing choice of commercially available monoclonal antibodies targeting FOXP3 is now available. Here, we report our experience in using two commonly used monoclonal FOXP3 antibodies on formalin- fixed paraffin-embedded sections of different organs including the cervix and vulva. The antibodies targeting different FOXP3 epitopes unexpectedly resulted in significantly different staining patterns. This phenomenon has not been previously reported and is likely to be an important observation.
