Human papilloma viruses do not play an aetiological role in Müllerian adenosrcomas of the uterine cervix
Everisto Mumba 1, Hasiena Ali 1, Diane Turton 1, Kum Cooper 2 and Wayne Grayson 1*
1 Division of Anatomical Pathology, University of the Witwatersrand, South Africa
2 Dept of Pathology, University of Vermont, United States
* To whom correspondence should be addressed. E-mail: wayne.grayson{at}live.com.
Accepted 21 May 2008
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Abstract |
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Aim: To determine if human papilloma viruses (HPVs) play a role in the histogenesis of adenosarcomas of the uterine cervix.
Methods: Nine archival cases of primary cervical adenosarcoma were studied. The HPV status of the 9 histologically proven tumours was investigated by non-isotopic in situ hybridisation (NISH) and the polymerase chain reaction (PCR). NISH was performed using digoxigenin labelled probes to HPV types 6, 11, 16, 18, 31 and 33. PCR employed GP5+/GP6+ primers to the HPV L1 gene.
Results: Neither the benign epithelial components nor the malignant stromal components of the 9 neoplasms harboured nuclear NISH signals for the HPV types investigated. Amplimers of the HPV L1 gene were not detected by PCR in any of the tumours studied.
Conclusion: HPVs do not appear to play an aetiological role in cervical adenosarcomas. This suggests that a different histogenetic pathway for this rare tumour type must exist.
