© 2003 BMJ Publishing Group & Association of Clinical Pathologists
ECHO
Tumour suppressor genes do not regulate the cell cycle
| The first 150 words of the full text of this article appear below. |
A study of the effects of hamartin and tuberin on cell growth suggests that tuberin acts as a tumour suppressor gene—but not by regulating the cell cycle. The researchers induced transient overexpression of hamartin and tuberin in cell cultures from a human kidney (HEK293) cell line by transfection. They used constructs of the TSC1 gene for hamartin and TSC2 gene for tuberin to transfect the cultures, separately and together, and constructs for three common TSC2 mutants They measured cell growth with two colour fluorescence activated cell sorting to analyse the percentage of cells in each growth phase.
TSC2, alone or with TSC1, produced a significant increase in G1 phase cells (41%, 39%) over the vector control (32%). So did a TSC2 mutation in exon 16 or 38 but not a C-terminus truncation mutation (41%, 40%, and 33%, respectively). TSC1 did not alter the percentage of G1 phase cells.
When the
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
- Full Text
- Full Text (PDF)
- Submit a response
- Alert me when this article is cited
- Alert me when eLetters are posted
- Alert me if a correction is posted
Services
- Email this link to a friend
- Similar articles in this journal
- Add article to my folders
- Download to citation manager
- Request Permissions
Citing Articles
Google Scholar
Bookmark with
Register for free content
The full back archive is now available for all BMJ Journals. Institutional subscribers may access the entire archive as part of their subscription. Personal subscribers will also have access to all content when logged in. Non-subscribers who register have free access to all articles published before 2006 right back to volume 1 issue 1. Register here to access the free archive of all BMJ Journals.
Don't forget to sign up for content alerts so you keep up to date with all the articles as they are published.
