© 2003 BMJ Publishing Group & Association of Clinical Pathologists
ECHO
Serum Ro52 antibody denotes connective tissue disease
| The first 150 words of the full text of this article appear below. |
A hitherto unrecognised subgroup of patients can now be identified, since the discovery of another, independent, serum marker for connective tissue diseases. Eighteen serum samples among more than 1700 tested routinely for antinuclear antibodies (ANAs), denoting connective tissue diseases, showed specificity solely for 52 kDa protein Ro52 and no cross reactivity with anti-SSA/Ro or SSB/La. None of them reacted with classic anti-SSA/Ro immunological methods—double immunodiffusion with thymus/spleen nuclear extract or natural Ro60, immunoblotting with natural or recombinant Ro60, and ELISA with natural SSA/Ro60 or recombinant Ro52 and Ro60 in unspecified proportions. Immunoblotting showed natural Ro60 as having just one 60 kDa protein band, which reacted exclusively with Ro60 monoclonal antibodies. However, 16 of the 18 sera reacted positively in ELISAs with equal amounts of recombinant Ro60 and Ro52, or Ro52 alone, or immunoblots with HeLa S100 substrate. Classic connective tissue disease was diagnosed for 12 of the 18 patients. The
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
- Full Text
- Full Text (PDF)
- Submit a response
- Alert me when this article is cited
- Alert me when eLetters are posted
- Alert me if a correction is posted
Services
- Email this link to a friend
- Similar articles in this journal
- Add article to my folders
- Download to citation manager
- Request Permissions
Citing Articles
Google Scholar
Bookmark with
Register for free content
The full back archive is now available for all BMJ Journals. Institutional subscribers may access the entire archive as part of their subscription. Personal subscribers will also have access to all content when logged in. Non-subscribers who register have free access to all articles published before 2006 right back to volume 1 issue 1. Register here to access the free archive of all BMJ Journals.
Don't forget to sign up for content alerts so you keep up to date with all the articles as they are published.
