© 2004 BMJ Publishing Group Ltd & Association of Clinical Pathologists
ECHO
New insights into early molecular events in colon cancer
| The first 150 words of the full text of this article appear below. |
For the first time, distinct gene expression profiles have been demonstrated in patients with colonic adenomas with low grade dysplasia. The detailed molecular analysis was made possible by laser mediated microdissection (LMM) of colonic crypts of normal and adenomotous colonic cryosections. This was followed by RNA arbitrarily primed polymerase chain reaction (RAP-PCR) and then use of cDNA expression arrays.
LMM was used to isolate colonic crypts of normal and adenomatous mucosa from six different patients. RAP-PCR was performed to screen mRNA populations and to generate hybridisation probes for cDNA expression arrays. Differential expression was then confirmed at the protein level by immunohistochemistry.
Upregulation of proliferation associated genes ras-oncogene related p21-rac1, mitogen activated protein kinase (MAPK) p38
and interferon 
receptor were found. Downregulation of apoptosis related genes (FAST kinase and p53) and thrombospondin 2 were also found. One of the pathways thought to be activated in the development of colonic tumours
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