Journal of Clinical Pathology 2007;60:945-947
SHORT REPORTS
Unrepresentative astrocytoma biopsy sampling is partly overcome by assessment of the MIB-1-labelled growth fraction
1 Departments of Pathology, Beaumont Hospital and the Royal College of Surgeons in Ireland, Dublin, Ireland
2 Departments of Clinical Neurological Sciences, Beaumont Hospital and the Royal College of Surgeons in Ireland, Dublin, Ireland
Correspondence to:
Dr Katherine Sheehan
Department of Pathology, Royal College of Surgeons in Ireland, Education and Research Building, Beaumont Hospital, Beaumont Rd, Dublin 9, Ireland; katherinesheehan@hotmail.com
Accepted 2 March 2007
| The first 150 words of the full text of this article appear below. |
Representative sampling of gliomas at biopsy is essential for correct assignation of histological grade and subsequent patient management. If sampling is unrepresentative, tissue devoid of mitoses or necrosis may be obtained and result in a falsely low glioma grade. The objective of the present study was to assess whether the MIB-1 labelling index of glioma tissue cores specifically constructed so as to replicate ‘unrepresentative’ astrocytoma biopsies would predict the real or actual glioma grade. Tissue microarrays were prepared from 134 samples of low-grade astrocytoma (LGA; WHO grade II), anaplastic astrocytoma (AA) and glioblastoma multiforme (GBM). Donor blocks were sampled in such a way as to avoid mitoses, necrosis and endothelial hyperplasia. Immunohistochemistry was performed using the Ki-67 (MIB-1) proliferation marker, and the percentage of MIB-1-positive cells per core was calculated. Mean MIB-1% values for LGA (n = 47), AA (n = 38) and GBM (n = 46) were mean (SD)
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