Journal of Clinical Pathology 2007;60:956-958
SHORT REPORTS
Improved cytochemical method for detecting Jordans’ bodies in neutral lipid storage diseases
1 Laboratory of Human Molecular Biology and Genetics, Catholic University of the Sacred Heart, Milan, Italy
2 National Institute of Molecular Genetics, Milan, Italy
Correspondence to:
Dr Roberto Colombo
Laboratory of Human Molecular Biology and Genetics, Catholic University of the Sacred Heart, Piazza Buonarroti 30, I-20123 Milan, Italy; roberto.colombo@unicatt.it
Accepted 20 December 2006
| The first 150 words of the full text of this article appear below. |
We describe an improved cytochemical procedure for detecting the presence of cytoplasmic lipid droplets (Jordans’ bodies) in blood cells from patients suffering from neutral lipid storage diseases (NLSDs). The method employs Oil red O (ORO), Nile red (NR) or, preferably, Bodipy (4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene) staining, coupled with DAPI (2-(4-amidinophenyl)-6-indolecarbamidine) staining of nuclei, to visualise clearly by fluorescence microscopy the presence of abundant neutral lipids (triacylglycerols and cholesterol esters) in granulocytes and monocytes. Using these reagents, we easily identified Jordans’ bodies in buffy coats from patients affected by Chanarin–Dorfman syndrome (NLSD with ichthyosis) and NLSD with progressive myopathy, a laboratory finding critical for the diagnosis of both inherited metabolic disorders. Due to their yellow-gold and green fluorescence arising selectively from neutral lipid binding and their water solubility that makes alcohol unnecessary for staining, NR and particularly Bodipy are superior to ORO for the specific detection of Jordans’ bodies in leucocytes.
Neutral lipid storage
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