A rapid technique for the determination of iron in serum, after the intravenous injection of iron-dextran, is presented.
Dithionite is used for the reduction of ferric to ferrous iron.
The use of a detergent solution enables protein precipitation to be omitted.
The iron reagent, 4: 7-diphenyl-1: 10-phenanthroline, and its ferrous complex are both soluble in the dilute detergent solution.
Lipaemic serum can be used without affecting the results.
The recovery of added ferric iron, as iron-dextran, is quantitative.
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