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THE DETERMINATION OF SERUM IRON AFTER THE INTRAVENOUS INJECTION OF IRON-DEXTRAN
  1. D. Webster
  1. Department of Medicine and Geriatric Medicine, the General Hospital, Sunderland

    Abstract

    A rapid technique for the determination of iron in serum, after the intravenous injection of iron-dextran, is presented.

    Dithionite is used for the reduction of ferric to ferrous iron.

    The use of a detergent solution enables protein precipitation to be omitted.

    The iron reagent, 4: 7-diphenyl-1: 10-phenanthroline, and its ferrous complex are both soluble in the dilute detergent solution.

    Lipaemic serum can be used without affecting the results.

    The recovery of added ferric iron, as iron-dextran, is quantitative.

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