Satisfactory spore preparations for testing hospital sterilizers can be made from B. stearothermophilus and comparatively simple methods for loading and drying the papers are considered to be valid. Each batch of spore papers should be calibrated by plotting dose-response curves for exposure to steam. A minimum standard in terms of the L.D.50 to moist heat is proposed and confidence limits suggested when such papers are used in pairs or small multiples.
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↵2 Present address: Central Public Health Laboratory, Colindale Avenue, N.W.9.
1 A report to the Medical Research Council Working Party on Steam Pressure Sterilizers. The work reported also formed part of a thesis submitted to the University of Cambridge for the degree of M.D.
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