Article Text

PDF

The use of tetramethylammonium hydroxide in the Zimmermann reaction
  1. V. H. T. James,
  2. Mary De Jong1
  1. Department of Chemical Pathology, St. Mary's Hospital, London

    Abstract

    The use of tetramethylammonium hydroxide (T.M.A.H.) in place of potassium hydroxide in the Zimmermann reaction has been investigated. Although various pure steroids have different colour equivalents, a comparison of the results of 17-ketosteroid and 17-hydroxycorticosteroid estimations on a series of urines showed that the differences for the two reagents were only small and may be ignored. Tetramethylammonium hydroxide has the considerable advantage over potassium hydroxide of stability and need not be prepared freshly. The correction procedures available are discussed.

    Statistics from Altmetric.com

    Footnotes

    • 1 Present address: Department of Chemical Pathology, St. Bartholomew's Hospital, London, E.C.1.

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.