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Techniques for the rapid and sensitive detection of penicillinase
  1. R. J. Holt,
  2. G. T. Stewart
  1. Queen Mary's Hospital for Children, Carshalton, Surrey

    Abstract

    Practical problems in the detection of penicillinase are discussed. A membrane technique for this purpose is described, with various modifications, suitable for screening large numbers of organisms and yet more sensitive than most methods.

    A one-stage membrane technique is adequate for detecting hydrolysis of penicillin G but a two-stage technique is required to demonstrate hydrolysis of some other penicillins. A gradient adaptation can be used for quantitative purposes.

    Staphylococci, coliforms, Proteus, and other organisms can be tested in this way for penicillinase formation; the method is also suitable for testing lysates, filtrates, and dialysates, provided independent provision is made for enzyme inducement.

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