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The assay of folic-acid activity of liver
  1. Myra C. Bennett,
  2. Valerie Berry,
  3. I. Chanarin,
  4. S. Ardeman
  1. M.R.C. Experimental Haematology Research Unit and Department of Haematology, Wright-Fleming Institute of Microbiology, St. Mary's Hospital Medical School, London

    Abstract

    Methods of preparing liver for the microbiological assay of folic-acid activity have been compared. Maximum values were obtained by incubating an alkaline homogenate of liver in the presence of ascorbic acid for five hours at 37°C. Most other procedures resulted in significant loss of folic-acid activity.

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