The polyacrylamide disc electrophoresis technique has been applied to the fractionation of `native' cerebrospinal fluid. Evidence is presented to show that there is an alteration in the protein profile if the fluid is subjected to a concentration stage. A minimum of 22 distinct protein zones have been detected in fluids from patients without structural changes in the nervous system. The method has also been applied to pathological fluids and the differences are discussed.
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