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Estimation of alpha-amino nitrogen in plasma and urine by the colorimetric ninhydrin reaction
  1. D. M. Matthews1,
  2. G. G. Muir2,
  3. D. N. Baron
  1. Department of Chemical Pathology, the Royal Free Hospital, London

    Abstract

    Methods are described for the estimation of plasma and urine α-amino nitrogen by the colorimetric ninhydrin reaction; in the method for urine, both ammonia and urea are separated by a rapid ion-exchange resin procedure. Two methods of estimation are given; one has the advantage of great simplicity, but involves the use of cyanide; in the other, cyanide is eliminated. Normal values are reported; they correspond well with those obtained by the gasometric ninhydrin method.

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    Footnotes

    • 1 Present address: Department of Chemical Pathology, National Hospital for Nervous Diseases, London, W.C.1.

    • 2 Present address: Department of Chemical Pathology, St. Bartholomew's Hospital, London, E.C.1.

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