Article Text

PDF

Method using ortho-tolidine for the quantitative determination of haemoglobin in serum and urine
  1. G. Parker Lewis1
  1. Southern General Hospital, Glasgow

    Abstract

    Following a study of the spectrophotometric properties of ortho-tolidine and its oxidation products, with particular attention to variation brought about by change in the hydrogen ion concentration, a method for the quantitative estimation of haemoglobin in serum and urine in which ortho-tolidine is substituted for benzidine in a peroxidase system is described. The method is designed to measure haemoglobin value within the range 0-125 mg. per 100 ml. Evidence is presented showing that it gives results equal in accuracy and reproducibility to a benzidine method in common use and for reasons stated is a more satisfactory technique for estimating the haemoglobin content of urine.

    Statistics from Altmetric.com

    Footnotes

    • 1 Present address: University Medical Unit, Stobhill General Hospital. Glasgow. N.1.

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.