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Developments in immunofluorescence: the need for standardization
  1. G. D. Johnson,
  2. E. H. Beutner2,
  3. E. J. Holborow
  1. Medical Research Council Rheumatism Research Unit, Canadian Red Cross Memorial Hospital Taplow, Nr. Maidenhead, Berkshire, England

    Abstract

    Difficulties experienced by the newcomer to the fluorescent antibody staining method largely arise from two causes: (1) the many modifications in the procedure which have been suggested over the last decade; (2) the variability of the basic reagent, fluorescent conjugate, in terms of potency and specificity. The ensuing problem of non-reproducibility is clearly an important factor for clinical laboratories now faced with increasing demands for routine tests involving immunofluorescence. In this paper a simple approach to standardization is described which, if adopted, would lead to a more efficient use of the method.

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    Footnotes

    • 2 Present address: State University of New York at Buffalo, Schools of Medicine and Dentistry, Buffalo, New York, U.S.A.

    • 1 This work was supported in part by U.S. P.H. Service Grant AM-10537-01.

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