A method for the determination of both direct and indirect serum bilirubin on the AutoAnalyzer is described which has a number of advantages over the automated method that is currently recommended. It gives total bilirubin results which are similar to those obtained with the equivalent manual method and direct bilirubin results which are usually about 10% lower.
The effect of haemolysis on estimations was investigated. It has little influence on the total, but significantly lowers the direct bilirubin results.
Simple methods for the preparation of true bilirubin standards and a stable control serum are described.
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