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An evaluation of current methods for the diagnostic histochemistry of amyloid
  1. John H. Cooper
  1. Department of Pathology, Pathology Institute, and Dalhousie University, Halifax, Nova Scotia, Canada

    Abstract

    Six current histological methods for demonstrating amyloid (crystal violet, thioflavine-T fluorescence, Congo-red staining and fluorescence, Sirius-red staining, and Congo- or Sirius-red birefringence) were applied in 25 cases of amyloidosis of various types and 47 pseudo-amyloid lesions. The results were compared and were correlated with those of ancillary histochemical tests and clinico-pathological data and each method's sensitivity and specificity for amyloid was evaluated. Thioflavine-T and, to a lesser degree, Congo-red fluorescence and Sirius-red staining proved very sensitive but not specific. Green birefringence with Congo or Sirius red was specific but not completely sensitive. The coexistence of Congo-red (and Sirius-red) staining and a positive DMAB-nitrite reaction occurred in all amyloid specimens and appeared specific for amyloid.

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