Cryoprecipitate antihaemophilic factor concentrate was prepared from fresh and 24-hour-old blood by quick and slow thaw methods. Recovery of factor VIII was greater by the slow thaw method and there was less loss into the supernatant plasma. Cryoprecipitate produced from fresh blood contained more factor VIII than that produced from 24-hour-old blood so that the most potent concentrate was produced from fresh blood by the slow thaw process. An adequate therapeutic preparation was, however, produced by the slow thaw method using 24-hour-old blood and it is suggested that this procedure could be adopted in order to supplement supplies.
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