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New method for the estimation of platelet ascorbic acid
  1. J. V. Lloyd,
  2. P. S. Davis,
  3. Harry Lander
  1. Department of Medicine, University of Adelaide, Adelaide, South Australia

    Abstract

    Present techniques for the estimation of platelet ascorbic acid allow interference by other substances in the sample. A new and more specific method of analysis is presented. The proposed method owes its increased specificity to resolution of the extract by thin-layer chromatography. By this means ascorbic acid is separated from other reducing substances present. The separated ascorbic acid is eluted from the thin layer and estimated by a new and very sensitive procedure: ascorbic acid is made to react with ferric chloride and the ferrous ions so formed are estimated spectrophotometrically by the coloured derivative which they form with tripyridyl-Striazine. Results obtained with normal blood platelets were consistently lower than simultaneous determinations by the dinitrophenylhydrazine (DNPH) method.

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