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Determination of individual bile acids in biological fluids by thin-layer chromatography and fluorimetry
  1. D. Panveliwalla,
  2. B. Lewis,
  3. I. D. P. Wootton,
  4. S. Tabaqchali
  1. Department of Chemical Pathology, Royal Postgraduate Medical School, London
  2. Medical Research Council Group on Intestinal Malabsorption, Department of Medicine, Royal Postgraduate Medical School, London

    Abstract

    A method is described for the separation and determination of individual bile acids in serum and in intestinal contents, employing thin-layer chromatography and fluorimetry. The mean recovery from intestinal juice was 94% and from serum 82%.

    Normal values for the individual bile acids of serum and examples of clinical applications of the method are given.

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