Abstract

The glyceride glycerol analysis depends, after saponification of triglycerides, on a linked enzymatic procedure using glycerokinase, pyruvate kinase, and lactate dehydrogenase: the final conversion of NADH to NAD⁺ is followed fluorimetrically. Twenty analyses can be performed per hour on the AutoAnalyzer; recoveries of added triglycerides ranged between 90 and 104%. In a mixed male and female group the normal range for glyceride glycerol was 2·5 to 15·5 mg/100 ml (0·2-1·4 mmol/l) fasting, and 2·5 to 18·0 mg/100 ml (0·2-1·6 mmol/l) postprandially using fresh serum. There was a significant rise postprandially in older men.