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A micro-method for peripheral leucocyte migration in tuberculin sensitivity
  1. K. Federlin1,
  2. R. N. Maini,
  3. A. S. Russell,
  4. D. C. Dumonde
  1. Division of Clinical Research, Kennedy Institute of Rheumatology, Hammersmith, London
  2. Division of Immunology, Kennedy Institute of Rheumatology, Hammersmith, London

    Abstract

    Inhibition of buffy layer peripheral leucocyte migration by tuberculin purified protein derivative (PPD) from micro-capillaries mounted in small tissue culture chambers correlates in all cases with a positive Mantoux test. This quick and reproducible test of cellular hypersensitivity in man requires only 5-15 ml of blood and is applicable for study in children and in clinical situations where repeated monitoring of cellular immunity may be required.

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    Footnotes

    • 1 Present address: Zentrum fuer Innere Medizin und Kinderheilkunde, Universitaet Ulm, Steinhoevelstr. 9, D 79 Ulm, Germany.

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