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Preparation of unfixed undemineralized bone sections: the Bright bone cryostat
  1. A. D. Pearse,
  2. D. L. Gardner
  1. Division of Experimental Pathology, Kennedy Institute, London

    Abstract

    A bone cryostat is described with which cold sections of unfixed, undemineralized calcified tissue can be prepared.

    The bone cryostat is suitable for the preparation of the sections required for the histochemical and immunofluorescent study of bone, for electron microprobe analysis, and for bone and joint autoradiography with soluble compounds.

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