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The adverse effect of bromelin in anti-D quantitation using the AutoAnalyzer
  1. H. H. Gunson,
  2. P. K. Phillips1,
  3. F. Stratton
  1. Regional Transfusion Service, Manchester

    Abstract

    With eight of 32 sera significantly higher estimations of anti-D concentration were obtained when test cells premodified with bromelin were used instead of bromelin as a reagent added to the reaction manifold. With one serum the difference in estimation, using the two techniques, revealed a ten-fold discrepancy. The consequences of these findings are discussed in relation to the use of the AutoAnalyzer for the quantitation of anti-D.

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    Footnotes

    • Postgraduate student, Department of Chemistry, University of Lancaster

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