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Observations on interactions between fluorescein-iso-thiocyanate conjugates and cells in vitro
  1. G. Sander1,
  2. J. P. Jacobs
  1. National Institute for Medical Research (Hampstead Laboratories), London

    Abstract

    During the course of some routine immunofluorescence studies `non-specific' staining occurred in the absence of free fluorescein and of any known impurities. The cause of such staining seems to be associated with phasic changes occurring at varying population doublings (`passages') in species-homologous cells subjected to serial cultivation.

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    Footnotes

    • 1 Present address: The Mathilda & Terence Kennedy Institute of Rheumatology, Bute Gardens, Hammersmith, London, W6 7DW

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