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An automated method for the microbiological assay of serum pyridoxal
  1. R. E. Davis,
  2. B. K. Smith,
  3. D. H. Curnow
  1. Department of Haematology, Royal Perth Hospital, Western Australia
  2. Department of Pathology, University of Western Australia

    Abstract

    A fully automated method for the measurement of serum pyridoxal has been developed. Acid phosphatase was used for dephosphorylation and precipitation of the serum proteins was not required. A chloramphenicol-resistant strain of L. casei was used as the test organism and this removed the need for sterilization. The method gives highly reproducible results, and is suitable for population and institutional studies.

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    Footnotes

    • 1 Please address requests for reprints directly to the author in the usual way. If a photocopy of the full paper is required, please send your request to the Publishing Manager, Journal of Clinical Pathology, BMA House, Tavistock Square, London WC1H 9JR, enclosing 25p. These reprint orders must be accompanied by this prepayment.