Article Text

PDF

Superiority of simplified assay for folate with Lactobacillus casei ATCC 7469 over assay with chloramphenicol-adapted strain
  1. Bernard A. Cooper,
  2. Erika Jonas
  1. Haematology Division, Royal Victoria Hospital, Montreal, Quebec, Canada
  2. McGill University Medical Clinic, Montreal, Quebec, Canada

    Abstract

    Direct assay of serum folate by mixing serum with a mixture of L. casei and medium without autoclaving was found to provide serum folate results similar to those by extraction of serum with heat. This procedure was found more reliable than that using the same technique with chloramphenicol and a strain of L. casei adapted to grow in this material. A portion of apparent folate activity assayed in haemolysates by such direct assay was found to be due to material other than folate. It is recommended that heat extraction of haemolysates be continued for determination of whole blood folate, or that an additional standardization of technique be included if direct assay is used.

    Statistics from Altmetric.com

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.