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Early detection of influenza virus in cell culture by means of immunofluorescence
  1. A. F. M. S. Rahman
  1. Public Health Laboratory and Department of Microbiology, Central Middlesex Hospital, London

    Abstract

    An indirect fluorescent antibody technique using human serum for the rapid detection of influenza A virus in monkey kidney cell cultures is described.

    Influenza A2 virus was isolated from 38 of 88 nose/throat swabs received from patients with suspected influenza during the winter of 1971-72.

    Thirty-two strains isolated in monkey kidney cell cultures were identified by immunofluorescence on the day haemadsorption was observed, 22 of them within a week of receipt of the specimen.

    Human antiserum was found to be very satisfactory for this purpose.

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