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A simple method for detecting mycoplasma infection of cell cultures
  1. G. B. Harnett,
  2. P. A. Phillips,
  3. E. M. Mackay-Scollay
  1. Department of Microbiology, Sir Charles Gairdner Hospital, Shenton Park, Western Australia
  2. State Health Laboratory Service, Sir Charles Gairdner Hospital, Shenton Park, Western Australia

    Abstract

    This paper describes the overnight Giemsa staining method for detecting mycoplasma infection of cell cultures. This simple method requires no special equipment and has practical application in most cell culture laboratories. This technique has been used conveniently to monitor stock cell cultures for mycoplasma infection over a 12-month period avoiding any contamination.

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