Article Text

PDF

Plasma alkaline phosphatase isoenzymes in hepatobiliary disease
  1. A. O. Afonja1,
  2. D. N. Baron2
  1. Department of Chemical Pathology, Royal Free Hospital, London

    Abstract

    By cellulose acetate or acrylamide gel electrophoresis it is possible to separate these alkaline phosphatase isoenzymes from serum: [anode] fast liver, slow liver, placenta/Regan, bone, intestine, bile [cathode]. Heat or chemical inhibition can confirm the differentiation.

    Normal adult serum always contains slow-liver isoenzyme, and sometimes bone isoenzyme: the latter is always present in serum of children. In hepatobiliary disease slow-liver isoenzyme was always increased: intestinal isoenzyme appeared in many cases of cirrhosis (of blood groups B and 0) but fast-liver and bile isoenzymes were occasionally seen in miscellaneous cases. The findings in other diseases included Regan isoenzyme in six out of 45 cases of malignant disease.

    Statistics from Altmetric.com

    Footnotes

    • 1 Present address: Department of Chemical Pathology, Lagos University Teaching Hospital, Nigeria.

    • 2 Correspondence to Professor D. N. Baron, Department of Chemical Pathology, Royal Free Hospital, London NW3 2QG.

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.