Two methods for the assay of rifampicin in serum are described. The first is a conventional plate diffusion method, measuring concentrations down to 0·02 μg/ml, and the second a chemical extraction followed by measurement of the inhibition of uptake of 14C-uridine by Staphylococcus aureus, which estimates in the range of 0·02 to 0·001 μg/ml. The methods were used to measure serum concentrations in man following doses of about 1050 mg and 75 mg rifampicin.
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