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A rapid, simple assay for digoxin
  1. Helena Greenwood,
  2. M. Howard,
  3. J. Landon
  1. Department of Chemical Pathology, St Bartholomew's Hospital, London

    Abstract

    An extremely rapid radioimmunoassay for digoxin is described which is precise over the range of concentrations required to determine whether, or not, a patient has digoxin toxicity. The assay is based on the use of 125-iodine-labelled digoxin and of a gel equilibration technique for the separation of antibody-bound and free digoxin. The results obtained compare closely with those by a conventional radioimmunoassay and the technique is sufficiently simple to enable its performance by relatively inexperienced laboratory staff.

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