Article Text

PDF

Intracellular immunoglobulins
  1. J. Burns,
  2. Maria Hambridge,
  3. C. R. Taylor
  1. Gibson Laboratories, Radcliffe Infirmary, Oxford

    A comparative study on three standard tissue processing methods using horseradish peroxidase and fluorochrome conjugates

    Abstract

    Formalin fixation followed by routine paraffin embedding allows the demonstration of intracellular immunoglobulin by a sandwich technique using either peroxidase or fluorescein isothyocyanate labelled antibody conjugates. The results compared favourably with those obtained using either the fresh frozen or the Sainte-Marie method for preserving tissue immunoglobulins. The formalin-paraffin method with peroxidase-labelled antibody has advantages for routine use. Morphology is excellent, preparations are permanent, and retrospective studies of stored paraffin-embedded tissue are possible.

    Some of the problems of labelled antibody studies are discussed.

    Statistics from Altmetric.com

    A comparative study on three standard tissue processing methods using horseradish peroxidase and fluorochrome conjugates

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.