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Research Article
Comparison of machine and manual staining of direct smears for acid-fast bacilli by fluorescence microscopy.
  1. J K Clancey,
  2. B W Allen,
  3. D T Rogers,
  4. L S Smith,
  5. V Aber,
  6. D A Mitchison

    Abstract

    Comparisons were made in Lusaka and in London between manual staining and staining in an automatic machine with auramine-phenol of direct smears of sputum and other types of specimen for acid-fast bacilli. No evidence was obtained of carry-over of acid-fast bacilli from positive to negative smears during machine staining. There was improved contrast between bacilli and the background in smears prepared with the machine.

    https://doi.org/10.1136/jcp.29.10.931

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