The methods used successfully to examine pus from abscesses of the central nervous system are described. The association between direct inoculation of intracranial pus into a liquid anaerobic culture medium and the isolation of viable bacteria is emphasised. Cultural methods for the recognition of the streptococci associated with brain abscess and methods for the assay of antimicrobial drugs in pus are presented. The role of gas liquid chromatography is discussed.
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