To determine the storage temperature needed for the preservation of standard sera for serodiagnostic tests, groups of sera were tested before and after storage at -70 degrees C and -20 degrees C for periods of up to five years. The temperature required depended on the nature of the test, but no definite advantage was found in storage at -70 degrees C. For complement fixation this temperature was inadequate and greatly inferior to liquid nitrogen. For immunofluorescence it had no apparent advantage over a temperature of -20 degrees C, and for some other tests it was no more than marginally superior.
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