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Improved double immunoenzyme labelling using alkaline phosphatase and horseradish peroxidase
  1. NJ Malik,
  2. ME Daymon
  1. Department of Histopathology, Royal Free Hospital, Pond Street, London NW3 2QG

    Abstract

    Alkaline phosphatase and peroxidase complexes were applied to tissue sections simultaneously to label two antigens. An azo-coupling method was used to demonstrate the alkaline phosphatase activity. The final result gave clear contrast between the two antigens and allowed the use of haematoxylin as the nuclear counterstain.

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