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Methods for the routine characterisation of isolates of Haemophilus
  1. PM Zadik
  1. Public Health Laboratory and Department of Microbiology, Royal Berkshire Hospital, London Road, Reading, Berkshire, RG1 5AN

    Abstract

    Methods and their evaluation are described for the routine characterisation of Haemophilus spp isolates using a test for dependence on V factor and a test for the conversion of delta-amino-laevulinic acid (ALA) to porphyrin in which the ALA is incorporated into a solid medium. A method is also described whereby the difference in the size of the inhibition zones around discs of ampicillin and of amoxycillin/clavulanate can be used to detect the production of beta-lactamase.

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