Article Text

PDF

A modified tetrazolium reaction for identifying malignant cells from gastric and colonic cancer
  1. KS Ibrahim,
  2. OAN Husain,
  3. L Bitensky*,
  4. J Cha Yen*
  1. Cytology Department, Charing Cross Hospital, London W6 8RF
  2. * Division of Cellular Biology, Kennedy Institute of Rheumatology, London W6 7DW

    Abstract

    When non-malignant cells were reacted for glucose-6-phosphate dehydrogenase activity, with neotetrazolium chloride as the indicator of the activity, oxygen competed with the neotetrazolium and nullified the reaction. In contrast, about 30% of the activity was retained in malignant cells, in sections and in smears, from cancer of the stomach or colon. This could provide the basis of a qualitative (black-or-white) functional test for distinguishing malignant cells in these conditions.

    Statistics from Altmetric.com

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.