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Enhanced luminescence enzyme immunoassay for factor VIII related antigen.
  1. H X Wang,
  2. J George,
  3. G H Thorpe,
  4. R A Stott,
  5. L J Kricka,
  6. T P Whitehead

    Abstract

    A sandwich enzyme immunoassay for plasma factor VIII related antigen has been developed which exploits a para-iodophenol enhanced chemiluminescent reaction to detect the horseradish peroxidase label. The assay entailed 15 min incubations with sample and with conjugate and had a detection limit of 0.12 mU. It showed good within batch precision (coefficient of variation = 2.95-5.8%) and results on a series of 57 specimens agreed with results obtained by immunoelectrophoresis (correlation coefficient = 0.97).

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