Article Text

PDF

Rapid diagnosis of gram negative urinary infections: identification and antimicrobial susceptibility testing in 24 hours.
  1. C M Dupeyron,
  2. G A Guillemin,
  3. G J Leluan

    Abstract

    A rapid method for diagnosing urinary tract infections, using identification and antimicrobial susceptibility testing that can be carried out in 24 hours, was devised. The method relies on direct inoculation of diluted urine (1/500) in the API 20 E and API ATB systems. Urine was simultaneously cultured on Columbia blood agar and on Drigalski agar to control the purity and for purposes of comparison. The results of this method and those obtained with a conventional method were compared by analysing 1352 urines. The results showed that all of the organisms were correctly identified using the conventional method, and susceptibility testing (rapid method) gave results that agreed with those of the classical method in 94% of cases, with major discrepancies in only 0.08% of cases. The rapid method applies only to monomicrobial infections.

    Statistics from Altmetric.com

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.