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Sensitive in situ hybridisation technique using biotin-streptavidin-polyalkaline phosphatase complex.
  1. F A Lewis,
  2. S Griffiths,
  3. R Dunnicliff,
  4. M Wells,
  5. N Dudding,
  6. C C Bird

    Abstract

    A sensitive in situ hybridisation technique, using a biotin-streptavidin-polyalkaline phosphatase complex detection system, was successfully applied to smears of fresh cultured cells, frozen sections, and formalin fixed paraffin processed tissue: the procedure was successful for DNA-DNA hybridizations using a variety of DNA probes. The detection method is rapid, reliable, and economical producing a purplish-blue precipitate at the site of hybridisation and clearly visible by low power light microscopy.

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