Article Text


Haemophilia A: carrier detection and prenatal diagnosis by linkage analysis using DNA polymorphism.
  1. E G Tuddenham,
  2. E Goldman,
  3. A McGraw,
  4. P B Kernoff
  1. Haemostatis Research Group, Clinical Research Centre, Harrow, Middlesex.


    Restriction fragment length polymorphisms (RFLPs) within or close to the factor VIII locus are very useful for genetic linkage analysis. Such RFLPs allow a mutant allele to be tracked in a family, segregating haemophilia A even when, as is usually the case, the precise mutation causing failure to synthesise factor VIII is unknown. To date two markers tightly linked to the factor VIII locus have been described, one of which is highly polymorphic and therefore informative in most kindreds. A significant crossover rate, however, does not make diagnosis absolute. Three intragenic RFLPs have been defined, which, taken together, are informative in about 70% of women, providing virtually deterministic genetic diagnosis.

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