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Evaluation of an enzymatic method for determining creatinine in plasma.
  1. H Crocker,
  2. M D Shephard,
  3. G H White
  1. Department of Biochemistry and Chemical Pathology, Flinders Medical Centre, Bedford Park, South Australia.

    Abstract

    An enzymatic kit method for the determination of plasma creatinine was optimised for use with a centrifugal analyser and its performance characteristics and practicability compared with an end point and a kinetic Jaffé-based method. The enzymatic method exhibited several advantages over Jaffé-based methods--namely, smaller sample size, rapid sample throughput (200 per hour), and improved specificity. Glucose, acetoacetate, and cefoxitin did not interfere with the enzymatic method, although bilirubin did cause a negative interference which depended on both creatinine and bilirubin concentrations. The enzymatic method has particular clinical application in neonates, diabetic ketotic patients, and those receiving cephalosporins.

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