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Comparison of western blot analysis and immunocytochemical detection of P-glycoprotein in multidrug resistant cells.
  1. M L Friedlander,
  2. D R Bell,
  3. J Leary,
  4. R A Davey
  1. Department of Clinical Oncology, Royal North Shore Hospital, St Leonards, New South Wales, Australia.

    Abstract

    A sensitive immunocytochemical technique was developed to detect a 170,000 dalton cell membrane glycoprotein (P-gp) in cell lines resistant to vincristine and vinblastine with varying degrees of resistance. P-gp was shown very clearly using the C219 monoclonal antibody and immunocytochemical detection with either antialkaline phosphate or peroxidase-antiperoxidase with silver gold intensification. There was good correlation between the results obtained with immunocytochemical detection of P-gp in single cells and Western blot analysis. The technique is easily performed and can detect P-gp in relatively small numbers of cells that Western blot analysis could miss and is suitable for clinical application.

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