Article Text

PDF

Feasibility of in situ hybridisation with chromosome specific DNA probes on paraffin wax embedded tissue.
  1. E P Arnoldus,
  2. E J Dreef,
  3. I A Noordermeer,
  4. M M Verheggen,
  5. R F Thierry,
  6. A C Peters,
  7. C J Cornelisse,
  8. M Van der Ploeg,
  9. A K Raap
  1. Department of Cytochemistry and Cytometry, Leiden University, The Netherlands.

    Abstract

    The feasibility was studied of in situ hybridisation using chromosome specific DNA probes on paraffin wax embedded normal and malignant tissues from different organs. Both isolated nuclei and 5 microns sections were used in in situ hybridisation experiments with biotinylated repetitive DNA probes specific for the centromeric regions of chromosomes 1 and 17. The hybridisation results were visualised with peroxidase-diaminobenzidine. The optimal pretreatments with sodium thiocyanate and pepsin were experimentally defined for the different tissues. Although interphase cytogenetics on paraffin wax embedded tissue is possible, the results indicate that it has its limitations, compared with investigations on fresh tumour tissue.

    Statistics from Altmetric.com

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.