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Detection and identification of Helicobacter pylori by the polymerase chain reaction.
  1. C Clayton,
  2. K Kleanthous,
  3. S Tabaqchali
  1. Department of Medical Microbiology, St Bartholomew's Hospital Medical College, West Smithfield, London.

    Abstract

    A polymerase chain reaction for the specific detection of Helicobacter pylori was developed using a primer pair derived from the nucleotide sequence of the urease A gene of H pylori. Specific amplification of a 411 base pair DNA fragment from all strains of H pylori tested was achieved. Ten organisms were detected using the PCR and the technique permitted direct detection of H pylori in clinical biopsy samples. PCR will be useful for both prospective and retrospective investigation of the aetiology and epidemiology of H pylori associated disease.

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